Hepatic physiology

Vivian Imbriotis | March 10, 2026

The liver has 5 main functions:

Synthesis

Storage

Metabolism

Immunological function

Bile (both nutritional and excretory)

Synthesis

90% of plasma proteins (albumin, factors, compliment)

Regulatory proteins (thrombopoetin, angiotensinogen, herceptin)

Nutrients (gluconeogenesis, ketones, non-essential amino acids)


Storage

Energy (glycogen and lipid droplets)

Fat soluble vitamins (ADEK + B12)

Minerals (ferritin, copper)

Blood (25% of total blood volume)


Metabolism

Glucose \(\leftrightarrow\) Glycogen

Triglycerides \(\leftrightarrow\) FFAs \(\to\) ketones

Amino acids \(\to\) ammonia + \(\alpha\)-keto acid

Lactate \(\to\) glucose

Ammonia \(\to\) urea

Phase 1 + phase 2 metabolism of xenobiotics


Immunological

Synthesis of compliment

\(\uparrow\)herceptin \(\to\ \ \downarrow\)ferroportin to deny iron to bacteria

Kuffner cells (liver macrophages) phagocytose opsonised pathogens

IgA and IgG in bile


Excretion (via bile)

Bile acids (95% enterohepatically cycled)

Cholesterol (1g/day)

Conjugated bilirubin

Drugs (ceftriaxone, apixaban, digoxin)

Heavy metals (lead, arsenic)

Composition

95% water, 5% solutes

Organic solutes: bile salts (40mM), cholesterol (4mM), conjugated bilirubin (2mM), lipids, de minimus protein. Bile salts formed from neutralization of bile acids; primary = made by hepatocytes, secondary = made by intestinal bacteria

Inorganic solutes: similar ionic profile to plasma


Formation of bile

95% of bile salts reabsorbed in terminal ileum

Recirculate to hepatocytes and actively transported into bile

H2O follows by osmosis ("bile-salt dependent")

Other substances (conjugated xenobiotics, bilirubin) are also actively excreted, with H2O following by osmosis ("bile-salt independent")

Bile ducts \(\to\) water added

Gall bladder \(\to\) ions + H2O reclaimed, concentrating bile


Functions of bile

Emulsify lipids: \(\uparrow\)SA \(\to \ \uparrow\)lipid absorption + \(\uparrow\)absorption of fat-soluble vitamins

Excrete xenobiotics, cholesterol, bilirubin

Immune functions (IgA and IgG)

Growth factors for enterocytes


Synthesis failure

Hypoalbuminaemia

Thrombocytopenia and macrocytic anaemia

Coagulopathy


Storage failure

Hypoglycaemia


Metabolism failure

Accumulation of lactate

Accumulation of ammonia (hepatic encephalopathy)

Accumulation of drugs normally hepatically conjugated

Accumulation of unconjugated bilirubin


Immunological

Loss of compliment \(\to\) susceptibility to sepsis


Excretion (via bile)

Accumulation of conjugated bilirubin

Accumulation of drugs normally excreted in bile

Sources

Protein deamination: amino acid \(\to\ \ \alpha\)keto acid + ammonia

Purine metabolism in skeletal muscle

Renal ammoniagenesis from glutamate


Distribution

98% ionized and largely ion trapped



Metabolism

Exclusively in liver

Ammonia + CO2 \(\to\) urea


Elimination

Urea mostly excreted renally

~15% enterohepatically cycled (metabolized by flora back to ammonia and returned by first-pass to the liver; lactulose diverts bacterial metabolism)