Laboratory assessment of hepatic function

Vivian Imbriotis | June 22, 2026

Albumin

  • Serum protein produced by liver
  • Normal = 35-50 g/L
  • Chronic hepatic synthetic failure \(\to\) hypoalbuminaemia
  • Half-life 20 days \(\to\) often normal in hyperacute liver failure
  • Part of Child-Pugh score in cirrhosis
  • Also lowered by inflammation (acute phase reactant), starvation, kwashiorkor, nephrotic syndrome

Prothrombin time

  • Time taken for clotting to occur after addition of tissue factor
  • Normal = 12-15 seconds (INR 0.8-1.2)
  • Liver generates all clotting factors except for factor VIII, VWF, calcium and tissue factor
  • But liver also generates antithrombotic protein C and S
  • Both impaired with synthetic failure
  • Therefore acute liver failure \(\to \ \uparrow\)PT that is disproportionate to in vivo coagulopathy (i.e. overestimates bleeding risk)
  • PT can also be elevated by vitamin K deficiency, anticoagulants (esp. warfarin), nephrotic syndrome, consumption (e.g. DIC)

Glucose

  • Primary metabolic fuel in human body
  • Normal = 5-10mmol/L
  • Normally, liver stores glucose as glycogen + can perform gluconeogenesis \(\to\) mobilize glucose \(\to\) "glucostat" function
  • Acute liver failure \(\to\) hypoglycaemia
  • In chronic liver failure, failure of insulin clearance \(\to\) chronic hyperinsulinaemia \(\to\) insulin resistance \(\to\) hyperglycaemia
  • Confounded by nutrition, diabetes mellitus, medications (e.g. exogenous insulin)

Ammonia

  • Product of amino acid and purine catabolism
  • Normal = <35\(\mu\)M
  • Normally, 100% of circulating ammonia \(\to\) liver \(\to\) urea cycle \(\to\) urea, which is 75% renally excreted and 25% excreted in stool (where some undergoes enterohepatic recirculation)
  • Failure of urea cycle due to metabolic liver failure \(\to\) ammonia accumulation
  • Causative agent in hepatic encephalopathy and cerebral oedema

Bilirubin

  • Product of haem catabolism; exclusively cleared by biliary route
  • Normal = <5\(\mu\)M
  • Normally unconjugated bilirubin \(\xrightarrow{\text{in liver}}\) conjugated bilirubin \(\to\) eliminated in bile
  • Metabolic failure \(\to \ \uparrow\)unconjugated bilirubin
  • Biliary obstruction / clearance failure \(\to \ \uparrow\)conjugated bilirubin
  • Takes days to accumulate \(\to\) typically normal in hyperacute, marker of chronic disease
  • Confounded by \(\uparrow\) haem breakdown (haemolysis, haematoma breakdown), enzyme deficiency (e.g. Gilbert syndrome)

Serum hepatic enzymes

  • AST (found in liver, heart, skeletal muscle) \(\to\) early but nonspecific marker of hepatocellular injury
  • ALT (predominantly hepatic) \(\to\) later, more specific marker of hepatocellular injury.
  • GGT (renal tubules, liver, biliary tract, pancreas) and ALP (small intestine, bone, placenta, biliary tract) \(\to\) nonspecific markers of biliary tract disease