Production and maturation
- Produced in foetal bone marrow
- Migrate to thymus, where they undergo positive and negative selection
- Undergo V(D)J recombination \(\to\) sister cells have randomly varying T-cell receptors
- Positive selection: cells that cannot bind MHC die.
- Negative selection: cells that become activated in thymus (by self-antigen) are killed. Cells that are weakly stimulated by self-antigen become T-reg cells.
- Remaining cells exhibit activity against non-self and tolerance to self
- They migrate to lymphoid tissues (nodes, spleen, tonsils)
Subtypes (determined in thymus)
- T-helper CD4+ cells
- T-regulatory CD4+ cells
- Cytotoxic CD8+ cells
Activation is stimulated by the T cell receptor binding to...
- An MHC1-antigen complex (CD8 cells)
- An MHC2-antigen complex (CD4 cells)
Costimulation is necessary for activation
- An antigen-presenting cell expresses B7, which binds CD28 on the t-cell
- No costimulation \(\to\) anergy
- CAR-T cells are modified to self-co-stimulate \(\to\) reduce anergy
Coinhibition (immune checkpoint)
- T-cell CTLA4 competes for B7 binding, inhibiting immune response \(\to\) anergy
- Immune checkpoint inhibitors block CTLA4 (or similar molecules) \(\to\) reduce anergy
Result of activation
- T-helper cells differentiate into Th1 (cell-mediated immunity promoting) or Th2 (humoral immunity promoting)
- Activated cell \(\to\) clonal proliferation
- Some clones become memory cells, others become active t-cells and leave lymphoid tissue
Functions of activated cells
- Th1 produces INF-\(\gamma\) \(\to\) activate macrophages and cytotoxic T cells
- Th2 costimulate B-cells \(\to\) enhanced antibody production
- Treg cells \(\to\) dampen immune response \(\to\) preserve tolerance to self, foetus, and commensural flora
- CD8 cells \(\to\) lyse human cells expressing non-self on MHC1